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Plasmid construction We constructed the signaling cascade split on two pMAP plasmids The first comprising the receptor ssTNT.R3, the membrane protein AtFLS-Trg-PhoB, and the second plasmid, comprising the transcription factor PhoB-VP64 and the reporter RUBY

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Further reading Cocaine: From Medical Marvel to Modern Menace in the United States, 18841920

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This is the highest-leverage signal