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Mature, well-differentiated monolayers of cells were then exposed to control (ultrapure water dissolved in culture medium, water/medium: 2% v/v) or e-cig smoke solution (containing 2 ppm diacetyl, water/medium: 2% v/v) on the apical side for 6 or 24 h (n = 3 subjects, each treatment was performed in duplicate)

Have you heard a smoker say Roll-your-owns are healthier than tailor-made cigarettes as a reason for switching over

CDC and the Food and Drug Administration (FDA) are working with state health departments to determine what might be causing the illnesses

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