D., Cheng, K., Hickey, G
GSTs and Virulent Bacteria In an early report, the accumulation of a GST transcript was observed in A
Plasmids and site-directed mutagenesis For the RNA interference-mediated knockdown of KEAP1, we used the lentiviral plasmid pLKO.1 from the TRC shRNA library (Sigma-Aldrich, Milan, Italy) expressing specific shRNA for 3UTR of human KEAP1 (#TRCN0000155340) or GFP as negative control [65]
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242 Additionally, UNA modification at the siRNA terminus improves siRNA stability and silencing efficacy while considerably reducing off-target effects, highlighting its potential in the development of new therapeutic siRNAs