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The IgG variants were purified using CaptureSelect C H 1 columns (Thermo Fisher) prior to size exclusion chromatography (SEC) to isolate monomeric fractions using a Superdex 200 Increase 10/300 column (Cytiva Life Sciences) with an KTA Avant 25 (Cytiva Life Sciences)

Histamine flows in from multiple sources: Pollen-triggered mast cell release Stress-triggered mast cell activation Histamine in food (aged cheese, fermented foods, leftover meat) Histamine produced by gut bacteria Other immune triggers (infections, exercise, temperature changes) At the bottom of the bucket, DAO and HNMT act as drains

12a), while the AS + FeNP treatment led to a substantial increase in TPC, with a 28.36% increase relative to the AS treatment (Fig