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GHK-Cu has a short systemic half-life (on the order of minutes ), but topically applied it penetrates the epidermal matrix where it persists for several hours

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8) Place the plate in the incubator or reader (if equipped with a heating device) and wait until a total pre-incubation time of 10 minutes (for assay temperature 22-25C) or 7 minutes (assay temperature 25-37C) is completed

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