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Regulation of tight junction permeability and occludin phosphorylation by rhoA-p160ROCK-dependent and -independent mechanisms

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Cobalt (II) solution was prepared by dissolving 20.3 g of Co (NO 3 ) 2 6H 2 O in 1 l of distilled water

The worms were then washed twice with 1 PBS and mounted onto 2% agarose pads for visualization using a ZEISS Axio Observer.Z1 (ZEISS, Oberkochen, Germany) inverted microscope equipped with a ZEISS AxioCam MRc color camera and a ZEISS Plan-Apochromat 20/0.8 WD 0.55 mm air immersion objective