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After the recovery of cellular redox homeostasis, KEAP1 translocates into the nucleus through Karyopherin 6 (importin 7), inducing NRF2 nuclear export to the cytosol, in a CRM1-dependent process through Nuclear Export Signal (NES) sequences present in NRF2

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After blocking, membranes were incubated with primary antibodies against SLC7a11 (1:1000, Abcam), GPX4 (1:1,000, Abcam), and PPAR (1:1,000

This step requires an enzyme called glutamate-cysteine ligase (GCL)

Targeted augmentation of nuclear gene output (TANGO) of scn1a rescues parvalbumin interneuron excitability and reduces seizures in a mouse model of dravet syndrome