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Using morphine remains essential in medical settings despite its risks in palliative care and acute pain relief

At 80% flask confluency, cells were harvested using DPBS for washing and incubated with 1.5 ml of 0.25% trypsin EDTA/DPBS and poly-vinyl-pyrrolidone for 34 mins at 37C to allow detachment

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He is one of the few characters to survive the film