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ARA-290 (Cibinetide) is a synthetic 11-amino-acid non-hematopoietic erythropoietin-derived peptide

Its involvement in sleep-associated hormonal rhythms makes DSIP particularly relevant for studying the relationship between circadian biology and endocrine signaling

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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen, 0.5 mM L- glutamme, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro