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Afterwards, the cells were washed 3 times with PBS, scraped in 500 L of a lysis solution, containing 100 mM ammonium bicarbonate (Sigma Aldrich, Germany) and 1% (w/v) sodium deoxycholate (Sigma Aldrich, Italy), and lysed on ice to prevent protein degradation by intracellular proteases
2.5.2 Hemolysate hemoglobin assay The ferricyanide in Drabkin solution oxidizes the +2-valent iron in the hemoglobin to +3 and converts the hemoglobin to methemoglobin
Cell 140 , 338348 (2010)
Nevertheless, astrocytes and other glia can also mediate detrimental effects in neurological disorders either through downregulation of essential functions or through gain of inappropriate functions such as promoting excess inflammation
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