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P.Trzciska-WencelJ.GoliskaP

All of these suffer from serious disadvantages: Sucrose gradients pose the problem of high osmolality The silica particles of Percoll may be engulfed by the potentially phagocytic cells [1] Both polysucrose and silica particles have a tendency to adhere to cell surfaces The extreme sensitivity of coelomocytes to lipopolysaccharide [1] makes Percoll less than ideal, in view of the significant levels of endotoxin in this medium OptiPrep offers some important advantages over these other gradient media for the fractionation of coelomocytes

Vicente AM, Ballensiefen W, Jnsson J-I

Greiner AN, Hellings PW, Rotiroti G, Scadding GK