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The resulting CL supernatant was then filtered through 0.45-m and 0.20-m syringe filters and either directly lyophilized for further experiments (that is, denoted as CL) or stored on ice for protein purification

It can be a great option, but many people hesitate when needles are involved, so it's not necessarily right for everyone

Interestingly, all other tested pathways (oxidative stress, DNA damage, and inflammatory stress) were not significantly activated by either AQ-QI or DEAQ-QI treatment (Supplementary Figure S5)

Townsend et al., 2003

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