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Extracellular vesicles and their roles in cancer progression

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GSH is synthesized in two steps, both ATP-dependent processes, catalyzed by the enzymes GCL (glutamate-cysteine ligase, aka -glutamylcysteine synthetase) and glutathione synthetase, and is regenerated by six enzyme-catalyzed reactions, known as the -glutamyl cycle

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We do this by taking a sample of blood and measuring the amount of drug in this known volume