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Significantly downregulated metabolites included glutamine, isoleucine, deamido-NAD + , and MG (PGF2/0:0/0:0), while upregulated metabolites included acylcarnitines, succinyladenosine, and leukotriene B4

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Tracheotomy was performed and the animals were allowed to breathe spontaneously through the tracheal cannula

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