Repeated aerosol-vapor JP-8 jet fuel exposure affects neurobehavior and neurotransmitter levels in a rat model
Protein concentrations were quantified using a BCA protein quantification kit according to the manufacturers protocol (Thermo Fisher Scientific, Rockford, U.S.A.), and equal amounts of proteins (~30 g) were resolved on 10%12.5% SDS-PAGE gels
Cells were seeded on low-attachment culture vessels (Corning) and cultured in mTeSR1 medium supplemented with 40 g ml 1 Matrigel, 1 ng ml 1 BMP4 (Invitrogen) and 10 M Rho kinase inhibitor (ROCK) (R&D) under hypoxic environment with 5% O 2
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