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The final concentrations of sertraline were 10 M for NCI-H1299, 7 M for NCI-H520, HCC15, NCI-H125 and NCI-H1975 and 2.5 M for Calu-6 cell line

2013;8(9):154855 IDF

2 followed doxorubicin treatment at 8 weeks of age as described in the NMN and chemotherapy treatment protocol above, while for the breeding trial in cisplatin-treated mice (Fig

The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)

EDTA) or buffers that may disrupt copper coordination

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