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Int J Mol Sci , 2022, 23: 560 Nichols-Vinueza DX, Delmonte OM, Bundy V, Bosticardo M, Zimmermann MT, Dsouza NR, Pala F, Dobbs K, Stoddard J, Niemela JE, Kuehn HS, Keller MD, Rueda CM, Abraham RS, Urrutia R, Rosenzweig SD, Notarangelo LD

RNA and protein were extracted from the remaining tissues

In the second step adding NADPH and glutathione reductase results in conversion of all GSSG to GSH which reacts subsequently with DTNB and fluorescent or colorimetric substrate to be quantified

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Despite being the primary approach, these treatments come with limitations, including significant adverse effects, high relapse rate, long duration of disease, and high price