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The flow rate was set at a constant 300 nL/min using an EASY-nLC 1000 ultra-high-performance liquid chromatography (UPLC) system

From these, we selected for estimation parameters that were not available from literature and that are most directly associated with the dynamics of the GSH and GSSG concentrations ([GSH] and [GSSG], respectively), for which we had available experimental data

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With a MTHFR gene defect, you have what is called a single nucleotide polymorphism (SNP)

The polymorphism is caused by a single point mutation (G to A nucleotide substitution), leading to a substitution of lysine for glutamate at position 487 (E487K) in the ALDH2 monomer [5]

This supports the potential of hydrogen as an effective adjunct to dopaminergic medications