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It was discovered 130 years ago in baker's yeast ( Saccharomyces cerevisiae )

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Because the test relies on the lifespan and turnover of red blood cells, any condition that alters red cell production, size, or survival can directly influence the result

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LPS administration caused characteristic morphological changes in lung sections, including severe immune cell infiltration, interstitial edema, alveolar wall thickening, and hemorrhage, indicative of successful ALI induction