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Assuming a sequential reaction, where the enzyme, GPX, must bind to both substrates, ROS and GSH, for the reaction producing GSSG to occur and letting ROS and GSH to be the leading and following substrates, respectively, is the the reaction rate for GSSG

& Feringa, B

Some research suggests that as the blood dries, the cells rupture and release their glutathione, which can then be measured using advanced techniques like liquid chromatography

As we age, however, we lose much of our ability to break down protein and form glutathione from individual aminos