There is no single correct amount more water simply spreads the same 2 mg of peptide across a larger volume, which makes small doses easier to measure accurately
A., DAntuono, A., Tiberi, E., Giordano, L., Pianini, T., & Romagnoli, C
Handling: Allow vials to warm to room temperature before opening to prevent moisture condensation
Light exposure represents a significant degradation pathway, with UV and visible light catalyzing oxidative damage to the peptide backbone and disruption of copper coordination
[PubMed: 1383331] 120
This suggests that copper-binding and ROS production is not restricted to a small number of pathological proteins but may be a common property of intrinsically disordered proteins