Since preferred nitrogen sources, such as ammonia and nitrogen were not present when proline was used as the sole carbon and nitrogen source, catabolite repression of the proline utilisation pathway ( PUT1 and PUT2 ) would not have occurred 19
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The biofilm matrix acts as a physical barrier to block antifungal penetration, and the sessile fungal cells exhibit metabolic dormancy and upregulated expression of drug efflux pumps and target-modifying genes, and the presence of drug-resistant cells are some of the main factors leading to immune evasion and weakened response to available drugs (Sahoo et al., 2025)
