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Phospholipids and their hydroxy and hydroperoxy derivatives, including 15-HETE-PE and 15-HpETE-PE, were analyzed by LC/MS using a Dionex Ultimate 3000 HPLC system coupled on-line to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) using a normal phase column (Luna 3 m silica C18(2) 100 , 150 2.0 mm, Phenomenex)

10.1007/7854_2015_5003 122 KemperT

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Key components of ROS-induced proapoptotic cascades including ASK-1, the Bcl-2 associated X-protein (Bax), p53, p38/pJNK/pERK, mTor, Akt, caspase-9, and caspase-3