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Some may notice reduced shedding within a few weeks, but visible improvement in thickness and strength may take several months

65 ) (Addgene, 52961) for CRISPRCas9 KO or into pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 66 (Addgene, 71236) for CRISPRi knockdown, as previously described 41

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Glutathione slows down the enzyme responsible for making melanin

56, 57 Data quality was assessed with phenix.xtriage tool 58 within the PHENIX suite of programs, 52, 59 while the phasing of experimental data for wild-type CstB was done by molecular replacement using PHASER 60 as implemented in PHENIX