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However, Ca 2+ overload (induced by pro-inflammatory cytokines) inhibits these flippases and activates scramblases, resulting in PS exposure on the cell surface (Lanser et al., 2021)

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Sample buffer containing SDS, glycerol, Tris-HCl pH 6.8, and bromophenol blue was added to each sample to the following final concentrations: 2% SDS, 10% glycerol, 50 mM Tris-HCl, and 0.02% bromophenol blue

Monitoring : Your vitals are monitored throughout the procedure

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