Briefly, a 1 ml reaction mix (500 nM TGR, 100 M NADPH, and 2050 M inhibitor) was incubated at room temperature for 30 min for fast inhibitors, or 2 h for slow inhibitors
Chamber temperatures were continuously monitored and regulated during the observation period to keep body temperatures at 36 C (Supplementary Table S1) at the start and end of the behavior testing
It was noted that the effects of UK-5099 treatment on GSH reduction and replication stress were less in 32D cells (Fig
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