Headache 1999
Similarly, ASD risk was higher for those in the middle third (2.14 times) and highest third (3.62 times)
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For cell proliferation assays with fold change in cell number, cells were cultured in replicates of 3 in 96-well plates at 1000 (suspension) or 150 (adherent) cells per well in 200 uL RPMI or DMEM base media under the conditions described in each experiment, and a separate group of 3 wells was also plated for each cell line with no treatment for an initial time point
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The downstream effects most consistently described in the literature: induction of collagen I and III, elastin, and decorin synthesis (Maquart et al., FEBS Letters , 1988), upregulation of VEGF and basic FGF transcripts, induction of antioxidant defense genes including SOD2 and metallothionein isoforms, and modulation of the MMP-2 and TIMP-1/2 balance toward non-fibrotic remodeling
