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After centrifugation at 1000 x g for 10 min, the supernatant (S1) was centrifuged for 20 min at 17.500 x g to yield the supernatant S2 and the pellet P2 (crude synaptosomal fraction)

Similarly, there were no changes in or differences between the LCLT or placebo groups of Cohort 2 in blood sodium, potassium, ALT, AST, total bilirubin, ALP, creatinine, fasting glucose, D-dimer, and ACE2 levels during the study (data not shown)

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