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The replacement of the media is essential for removing the CuCl 2 and Cu(OAc) 2 interference before assaying the LDH levels as they are shown to reduce the fluorescence signal read-out within treated wells

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The anagen phase ratio, calculated as the number of hair follicles in the anagen phase out of all follicles, was measured on days 3, 6, and 9 of culture to evaluate the maintenance rate of the growth phase

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