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2 delta CT method was utilized to calculate the relative gene expression of target genes and -actin were used as reference genes

PubMed Kang, E.A., Han, Y.M., An, J.M., Park, Y.J., Sikiric, P., Kim, D.H., Kwon, K.A., Kim, Y.J., Yang, D., Tchah, H., & Hahm, K.B

Notably, hypoxic TMEs induce the overexpression of hypoxia-inducible factor 1-alpha (HIF-1), which in turn promotes the transcription of lipid-associated genes, such as lipid transporters, FASN, and sterol regulatory element-binding protein 1 (SREBP1)

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Glycine protects partial liver grafts from Kupffer cell-dependent ischemia-reperfusion injury without negative effect on regeneration