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The cells were cultured in DMEM (Gibco, Gaithersburg, USA) supplemented with 10% fetal bovine serum (Gibco, Gaithersburg, USA), 100 U/mL penicillin, and 100 g/mL streptomycin
(PubMed) Sena CM, Cipriano MA, Botelho MF, Seica RM
First treatment has partially diminished the sharp pain while in full extension
L-theanine - a naturally occurring amino acid that offers a calming effect without leading to drowsiness