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The landmark 1998 study by Raun et al

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Transplanted patients who smoke face an increased likelihood of developing vascular complications, such as hepatic artery thrombosis, which can severely compromise graft function and patient survival [108]

There are also practical considerations

The resulting aMD5-Lys-NH-resin was equilibrated with 20% N , N -diisopropylethylamine in N -methylpyrrolidone and treated with 0.2 M NHS-fluorescein or NHS-biotin in N , N -diisopropylethylamine in N -methylpyrrolidone