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A total of 23 male patients with androgenetic alopecia (AGA), with a Hamilton-Norwood baldness scale score of 3-5 and an age range of 23-46 (median age 34.2), were recruited for this study, along with 5 healthy male controls (age range of 22-38, median age of 27.5)

The resulting trimmed and de-duplicated reads were then aligned to the Rattus norvegicus Rnor6.0 reference genome, available on ENSEMBL, using STAR aligner v.2.5.2b4 25 , producing BAM files

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