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USA 95 , 51725177 (1998)

Some researchers add an extra 0.1mL of bacteriostatic water to compensate, accepting a slightly lower concentration (3.23mg/mL instead of 3.33mg/mL) in exchange for recovering nearly all the peptide

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however, they also cause stress to the animal due to anesthesia or animal handling, defeating the purpose of studying metabolism at steady state (97)

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