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After 24 h, medium was replaced with DMEM/FBS, and cells were transfected with 9 g of pOG44 plasmid and 1 g of the respective pcDNA 5 vector using 30 l of Lipofectamine 2000 (Thermo Fisher Scientific) in OptiMEM (Thermo Fisher Scientific) according to the manual

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A double-edged sword with a therapeutic target: Iron and ferroptosis in immune regulation

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GH and IGF-1 are surrogate biomarkers they tell you the drug engages its axis, not that it produces any clinical benefit or is safe over the long term

CGRP represents the most intriguing biological mechanism, linking the nervous system to bone repair, but direct therapeutic application is complicated by its role in pain signaling and the current clinical use of CGRP-blocking agents for migraine treatment