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The number of samples was determined by the Universal Population Volume Known Sampling Calculation Formula (n = Nz 2 pq/d 2 (N 1) + z 2 pq)

Mutation of His 18 to Ala abolished activity, consistent with the central role of His 18 in catalysis

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Similarly, the acetate-producing genus Dubosiella facilitates butyrate synthesis through microbial cross-feeding, as supported by its strong positive correlation with butyrate levels 48,49

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