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The results of a clinical trial in older men

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These biological and environmental discrepancies limit external validity and severely constrain the generalizability of preclinical findings to real-world clinical settings

2.2 Primary culture of myocytes and thermal stress trial 2.2.1 Primary culture of myocytes Juvenile fish, each weighing approximately 5 g, were dissected under sterile conditions to obtain muscle tissue, which was then cut into pieces and transferred to a 24-well cell culture plate

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