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When in doubt, always consult your physicianand remember that proper training and sterile technique are key to safe administration.

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Different medications have different tolerance windows for room temperature exposure

PMID 25977272

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A study that looked at 0.5-1mg/kg/day leucovorin in patients with cerebral folate deficiency (and related neurological symptoms) found that 90% of those supplemented achieved normal 5-methyltetrahydrofolate cerebral spinal fluid concentrations