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The number of macroscopic colonies in the spleen was counted after 12 days, following fixation in Bouins solution [2]

NSBSO showed GSH-dependent cytotoxicity and depletion of intracellular GSH

Your provider will set realistic expectations based on your labs

A modified 18 approach was used to measure AChE activity, 50 l of 0.5 mM DTNB in 1% sodium citrate, 200 l of 0.5 M phosphate buffer (KH2PO4/K2HPO4

A., Mignion, L., Desmet, C., Gourgue, F., Jonas, J

HFHC group mice were additionally treated with caffeine (75 mg/kg) or EGCG (100 mg/kg) for 6 weeks, using obeticholic acid (OCA,10 mg/kg) as a positive control group