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Additionally, chemoaffinity protocols such as immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO 2 ), although widely used in phosphoproteomics, lack site selectivity for phosphorylation at serine (pS), threonine (pT) or tyrosine (pY) 8,9 and exhibit a sequence bias in favor of peptides rich in aspartic (D) and glutamic (E) acid

When exogenous pure compounds were spiked to fresh blood (Sample Type 1), recovery rates were below 100%: NAD + at 92.5%, NADH at 10%, NADP + at 80% and NADPH at 60%

(5) Subjects who have donated blood or lost more than 400 mL of blood within the past 3 months, or who plan to donate blood during the study period

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In research contexts, it is studied as a discrete pentadecapeptide with reproducible physicochemical properties, allowing its use in biochemical, cellular, and preclinical experimental systems

Thus the results of this test will help to create awareness about G6PD deficiency in the society