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c , e , Exponential-phase ( c ) and stationary-phase ( e ) cultures were treated with and without 40 M KL1 and 2 g/mL Rif for 24 h, followed by a wash step and plating to enumerate surviving bacteria
The rate-limiting step in GSH synthesis is the availability of cysteine, which is transported into the brain from the blood via the cystine/glutamate antiporter system xc-
Drugs used for assays were: PTL (Enzo Life Sciences, Exeter, UK) at 1-10 M, N-acetyl cysteine (NAC, Sigma-Aldrich) at 15 mM and 30 M, and sulfasalazine (SSZ, Sigma-Aldrich) at 300 M, all prepared in suspension medium
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