LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling
Regular Glutathione is not easily absorbed by the skin
Answer: Investigational peptides demand careful, individualized oversight, which a rushed visit cannot provide
While widely used in many countries, the exact composition can vary by market
Cells were washed three times in 500 l PBS, lysed with 0.2% Triton X-100 at 37 C for 10 min and plated on LB agar
Subsequently, GSH ester (100 M) was added to replenish the intracellular GSH loss, which led to recovery of GSH to the basal level in the next 3 min, as recorded by RT (Fig