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In vitro experiments confirmed that A 25-35 treatment significantly inhibited the activation of the NRF2/SLC7A11/GPX4 signaling pathway in HT22 cells, upregulated the expression of Fe 2+ , TFR1, ACSL4, MDA, and GSSG, and decreased the expression of FTH1, SOD, and GSH, as well as the GSH/GSSG ratio, resulting in dysregulated neuronal iron metabolism, impaired amino acid antioxidant systems, mitochondrial dysfunction, and ferroptosis, which ultimately promoted neuronal injury

73 (2), e12812 (2022)

Self-directed combination therapy is strongly discouraged due to safety concerns

The mixed solution should be protected from light, ideally using amber vials or storing in a dark location within the refrigerator

& Meriga, B

Studies on the brain show it may affect mood-related chemicals like serotonin and dopamine, which could help protect the nervous system