Only one paper showed that glyceraldehyde-3-phosphate dehydrogenase, a key enzyme in glycolysis, is involved in iron metabolism as a transferrin-binding protein, independent of its canonical role in glycolysis [86]
Accordingly to manufactures instructions, the assay was performed as following: after incubating the ASCs with the SHBG, culture media was collected and centrifuged in order to remove remaining debris
Such insights guide rational development: one can prioritize compounds that hit multiple synergistic targets or design combination therapies that harness beneficial polypharmacology while minimizing promiscuous interference
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Reputable vendors use insulated packaging and cold chain shipping for temperature-sensitive compounds
10.1186/s40478-019-0737-z [DOI] [PMC free article] [PubMed] [Google Scholar] Lewerenz J., Maher P
