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Notably, similar inhibitions were observed both with the wild type enzyme and its cysteine mutants, suggesting that thiol conjugation is dispensable for GST inactivation by auronofin in contrast to other described targets 103

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Furthermore, the observed absence of adverse effects across multiple organ systemsincluding the brain, heart, lungs, liver, kidneys, and gastrointestinal tractstrengthens the evidence for its safety profile and supports its systemic cytoprotective potential [129,130,131,132,133,134,135,136,137,138,139,140,141,142,168]