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In addition, we used cubic restricted splines (with knots at 10, 50, and 90% percentiles) on IGF-1 as continuous variable to model a possible nonlinear association with mortality

After centrifugation (1200 g, 10 min), the supernatant was discarded, and the resulting cell pellet was resuspended in culture medium supplemented with 5% of (FBS) (F4135, Sigma- Aldrich, Pozna, Poland) and seeded into cell culture flasks

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It leads to an aversion to continuing with the present activity, decreased commitment to the tasks, and difficulties in concentration, memory, and overall mental performance [92]