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We exposed cell lines that had either Keap1, Nrf2 or Srxn1 tagged with green fluorescent protein (GFP) to a concentration range of DEM and monitored the response of these Nrf2 pathway components using high throughput confocal imaging (Fig

The next day, the coverslips were lifted by adding a small volume (~200l) of TBS-Gelatine under the coverslip and transferred back to the 24-well plate

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