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Cells were then incubated with a primary antibody for 12 h at room temperature, followed by the corresponding fluorophore-conjugated secondary antibody (Alexa Fluor) for 45 min and DAPI staining (1 g ml 1 DAPI

High globulin levels may signal severe iron deficiency

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2ce and Extended Data Fig

On balance, any improvements in plasma homocysteine from moderate coffee consumption (approximately 150ml per day of the filtered preparation) may be due to a synergy of the beneficial polyphenols ingested at optimal dietary levels acting on biochemical pathways that are yet to be elucidated