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Sample preparation Tissue sample Wash fresh tissues with PBS for twice, then add 0.1 g of animal/plant tissue into homogenizer (the homogenizer has been rinsed with Reagent I and placed on ice before use)

viii Lang C, et al

Wang Q, Zhang Y, Ma Y, Wang M, Pan G

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When systemic glutathione levels are low, the resulting unchecked oxidative stress acts as a constant irritant to mast cells, keeping them in a hyper-reactive state

Alexandria J Med 54:287293