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For ROS detection, peptide conjugate solution (400 M) and 10 M DCFH were mixed in a 1:1 (v/v) ratio, yielding a final peptide concentration of 200 M in a total volume of 100 l per well in 96-well plate

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iii) when the rats underwent early treatment at 20 min after L-NAME administration: before medication ( a ) and after medication ( b , c , d , e , f , and g ), immediately after ( b ), at the days 1 ( c ), 2 ( d ), 7 ( e ), 14 ( f ), and 28 ( g ) L-NAME time